Mutagenic PCR.

Mutagenic, Anti-Mutagenic and Cytotoxic Activities of Artediffusin (Tehranolide), in vitro, extracted from Artemisia diffusa

Background: Artediffusin is a sesquiterpene lactone with an endoperoxide group which has been isolated from Artemisia diffusa. Artemisia has always been of great botanical and pharmaceutical interest and is useful in traditional medicines for the treatment of a variety of diseases and complaints. The aims of the present study were to evaluate the cytotoxic, mutagenic and anti-mutagenic activiti...

Summary of Mutagenic Toxicity

Mutagenic Action of Mitomycin

HE antibiotic mitomycin C (MC) is mutagenic in some strains of Escherichia Tcoli in which it also increases the frequency of recombination (IIJIMA and HAGIWARA 1960). Studies on the effects of MC in Ustilago and Saccharomyces indicate that it is nonmutagenic in these organisms although it does increase mitotic crossing over (HOLLIDAY 1964). SUZUKI (1965) has found that MC increases crossing ove...

PCR Based Random Mutagenesis Approach for a Defined DNA Sequence Using the Mutagenic Potential of Oxidized Nucleotide Products

Oxidizing conditions have not been explored well for the in vitro random mutagenesis in directed evolution. The mutagenic potential of diverse range of oxidized products is well reported in literature. Incorporation of errors during PCR in the presence of oxidized nucleotides can be a very effective alternative to error prone PCR as the transversion mutation frequency is higher in the former ca...

Mutagenic activity of diazoacetylglycine derivatives.

Some diazoacetyl derivatives of glycine known as antitumor and immunosuppressive agents have been found to be powerful mutagens of the base substitution type.